Plasmid

Part:BBa_I713030:Design

Designed by: Zhenyu Shi   Group: iGEM07_Tsinghua   (2007-10-26)


pACYC-Lux


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1993
    Illegal XbaI site found at 1725
    Illegal XbaI site found at 4474
    Illegal PstI site found at 2012
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1993
    Illegal NheI site found at 3633
    Illegal PstI site found at 2012
    Illegal NotI site found at 2030
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1993
    Illegal BglII site found at 2186
    Illegal BamHI site found at 1987
    Illegal XhoI site found at 2235
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1993
    Illegal XbaI site found at 1725
    Illegal XbaI site found at 4474
    Illegal PstI site found at 2012
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1993
    Illegal XbaI site found at 1725
    Illegal XbaI site found at 4474
    Illegal PstI site found at 2012
    Illegal NgoMIV site found at 1740
    Illegal NgoMIV site found at 2205
    Illegal AgeI site found at 2447
    Illegal AgeI site found at 3719
    Illegal AgeI site found at 4042
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 878


Design Notes

Digest with NcoI and BamHI


Source

pACYCDuet-1 pGL3-Basic

References